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| Item Type: | Article |
|---|---|
| Title: | Ultrastructural analysis of DNA complexes during transfection and intracellular transport |
| Creators Name: | Cartier, R. and Velinova, M. and Lehman, C. and Erdmann, B. and Reszka, R. |
| Abstract: | We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2-1-{my}m aggregates composed of 30-50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates. |
| Keywords: | Gene Transfer, Polyplexes, Transfection Mechanism, NLS |
| Source: | Journal of Histochemistry & Cytochemistry |
| ISSN: | 0022-1554 |
| Publisher: | Histochemical Soc |
| Volume: | 51 |
| Number: | 9 |
| Page Range: | 1237-1240 |
| Date: | September 2003 |
| Official Publication: | http://www.jhc.org/cgi/content/abstract/51/9/1237 |
| PubMed: | View item in PubMed |
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