Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury

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Item Type:	Article
Title:	Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury
Creators Name:	Makara, J.K., Rappert, A., Matthias, K., Steinhaeuser, C., Spaet, A. and Kettenmann, H.
Abstract:	Chloride channels are important for astrocytic volume regulation and K(+) buffering. We demonstrate functional expression of a hyperpolarization-activated Cl(-) current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na(+) and K(+) were substituted with NMDG(+) and Cs(+) in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl(-) current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd(2+). The current was not detectable in astrocytes from ClC(-2) knockout mice, indicating that the ClC(-2) chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site.
Keywords:	Astrocytes, Brain, Brain Injuries, Cadmium, Cell Membrane, Cerebral Cortex, Cesium, Chloride Channels, Chlorides, Developmental Gene Expression Regulation, Glial Fibrillary Acidic Protein, Gliosis, Green Fluorescent Proteins, Hippocampus, Knockout Mice, Luminescent Proteins, Membrane Potentials, Organ Culture Techniques, Transgenic Mice, Animals, Mice
Source:	Molecular and Cellular Neuroscience
ISSN:	1044-7431
Publisher:	Academic Press
Volume:	23
Number:	4
Page Range:	521-530
Date:	August 2003
Official Publication:	https://doi.org/10.1016/S1044-7431(03)00080-0
PubMed:	View item in PubMed

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