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Interleukin-10 promoter polymorphism IL10.G and familial early onset psoriasis

Item Type:Article
Title:Interleukin-10 promoter polymorphism IL10.G and familial early onset psoriasis
Creators Name:Hensen, P. and Asadullah, K. and Windemuth, C. and Rueschendorf, F. and Hueffmeier, U. and Staender, M. and Schmitt-Egenolf, M. and Wienker, T.F. and Reis, A. and Traupe, H.
Abstract:Background: The anti-inflammatory cytokine interleukin (IL)-10 is considered to play a major role in the pathophysiology of psoriasis, which is characterized by an IL-10 deficiency. Systemic administration of 1L-10 has been shown to be an effective therapy for psoriasis. The 1L-10 promoter region contains a highly polymorphic microsatellite (IL10.G) and in a recent case-control study the IL10.G13 (144 bp) allele was found to be associated with familial early onset psoriasis (type 1 psoriasis) having a susceptible effect. Objectives: As it is essential in multifactorial diseases to replicate findings before definite conclusions can be drawn, we decided to perform a follow-up study and to follow a genetic approach analysing allele transmission in families with a positive family history of psoriasis. Methods: We studied 137 nuclear families (trio-design) comprising 456 individuals and genotyped the IL10.G marker. For comparison we also genotyped the microsatellite tn62 as a reference marker of the major psoriasis susceptibility locus on chromosome 6p21 (PSORS1). In the present study allele transmission was evaluated using the family-based association test (FBAT) and GENEHUNTER 2.0 based on the transmission/disequilibrium test. Results: The G13 allele (144 bp) had a frequency of 24%, was present in 88 families and clearly showed an even transmission (FBAT, P = 0.753). In contrast, allele 3 (IL10.G9) (136 bp) had a frequency of 39%, was present in 110 families and was transmitted in 43 trios and remained untransmitted in 67 trios (FBAT, P = 0.026), thus showing preferential nontransmission. For the HLA-linked tn62-marker we obtained a P-value of 0.000 27 for allele 4 in the same study group. Conclusions: In conclusion, we failed to confirm the susceptible effect of the G13 allele, but provide the first data for a protective effect of allele 3 (IL10.G9) for familial psoriasis. Our results suggest that the IL10.G polymorphism is not a major locus, but acts as a minor locus.
Keywords:Complex Diseases, Cytokine, Disease Susceptibility, Genetics, Promoter Polymorphism, Psoriasis
Source:British Journal of Dermatology
Publisher:Blackwell Publishing
Page Range:381-385
Date:August 2003
Official Publication:https://doi.org/10.1046/j.1365-2133.2003.05411.x
PubMed:View item in PubMed

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