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IL-1 β-dependent activation of NF-κB mediates PGE(2)release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase

Item Type:Article
Title:IL-1 β-dependent activation of NF-κB mediates PGE(2)release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase
Creators Name:Catley, M.C. and Chivers, J.E. and Cambridge, L.M. and Holden, N. and Slater, D.M. and Staples, K.J. and Bergmann, M.W. and Loser, P. and Barnes, P.J. and Newton, R.
Abstract:Prostaglandin (PG) E(2) release is induced in pulmonary A549 cells by the NF-{kappa}B-activating stimuli interleukin-1{beta} (IL-1{beta}) and phorbol 12-myristate 13-acetate (PMA). Adenoviral over-expression of I{kappa}B{alpha}ΔN, a dominant NF-{kappa}B inhibitor, prevents NF-{kappa}B-dependent transcription and was used to qualify the role of NF-{kappa}B in the release of PGE2. I{kappa}B{alpha}ΔN repressed IL-1{beta}-induced, but not PMA-induced, cycloxygenase-2 (COX-2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF-{kappa}B in the co-ordinate induction of COX-2, mPGES and in the corresponding release of PGE2 by IL-1{beta}. However, other pathways are primarily responsible for PGE2 release induced by PMA.
Keywords:Cyclooxygenase-2, Prostaglandin GH Synthase, Nuclear factor {kappa}B, Prostaglandin E Synthetase
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:547
Number:1-3
Page Range:75-79
Date:17 July 2003
Official Publication:https://doi.org/10.1016/S0014-5793(03)00672-0
PubMed:View item in PubMed

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