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| Item Type: | Article |
|---|---|
| Title: | Structure of the phenylalanyl-tRNA synthetase genes from Thermus thermophilus HB8 and their expression in Escherichia coli |
| Creators Name: | Kreutzer, R. and Kruft, V. and Bobkova, E.V. and Lavrik, O.I. and Sprinzl, M. |
| Abstract: | A 4459 bp long BamHI restriction fragment containing the two genes for the Thermus thermophilus HB8 phenylalanyl-tRNA synthetase was cloned in Escherichia coli and its nucleotide sequence was determined. The genes pheS and pheT encode the alpha- and beta-subunits with a molecular weight of 39 and 87 kD, respectively. Three conserved sequence motifs typical for class II tRNA synthetases occur in the alpha-subunit. Secondary structure predictions indicate that an arm composed of two anti-parallel alpha-helices similar to that reported for the E.coli seryl-tRNA synthetase may be present in its N-terminal portion. In the beta-subunit clusters of hydrophilic amino acids and a leucine zipper motif were identified, and several pronounced alpha-helical regions were predicted. The particular arginine and lysine residues in the N-terminal portion of the beta-subunit, which were found to participate in tRNA binding in the yeast and E.coli PheRSs, have their counterparts in the T.thermophilus protein. The 5'-portion of an open reading frame downstream of pheT was found and codes for a yet unidentified, extremely hydrophobic peptide. The pheST genes are presumably cotranscribed and translationally coupled. A novel type of a putative transcriptional terminator in Thermus species was identified immediately downstream of pheT and other Thermus genes. The genes pheS and pheST were expressed in E.coli. |
| Keywords: | Amino Acid Sequence, Base Sequence, Molecular Cloning, Escherichia coli, Bacterial Gene Expression Regulation, Molecular Sequence Data, Phenylalanine-tRNA Ligase, Plasmids, Restriction Mapping, Thermus thermophilus |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 20 |
| Number: | 16 |
| Page Range: | 4173-4178 |
| Date: | 25 August 1992 |
| Official Publication: | https://doi.org/10.1093/nar/20.16.4173 |
| PubMed: | View item in PubMed |
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