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Analysis of DLC-1 expression in human breast cancer

Item Type:Article
Title:Analysis of DLC-1 expression in human breast cancer
Creators Name:Plaumann, M. and Seitz, S. and Frege, R. and Estevez-Schwarz, L. and Scherneck, S.
Abstract:The chromosome region 8p12-p22 shows frequent allelic loss in many neoplasms, including breast cancer (BC). The DLC-1 gene, located on 8p21-p22, might be a candidate tumor suppressor gene in this region. To evaluate the involvement of DLC-1 in breast carcinogenesis we studied DLC-1 mRNA expression in a panel of 14 primary human BC and the corresponding normal breast cells as well as 8 BC cell lines. Low levels or absence of DLC-1 mRNA were observed in 57% of primary BC and 62.5% of BC cell lines, respectively. We could not find any correlation between DLC-1mRNA expression and deletions at the DLC-1 locus. Transfection of the gene into DLC-1 deficient T-47D cells raised the DLC-1 mRNA level and resulted in inhibition of cell growth and reduced colony-forming capacity. Our results indicate a role of DLC-1 in BC carcinogenesis.
Keywords:Breast cancer, DLC-1 expression
Source:Journal of Cancer Research and Clinical Oncology
ISSN:0171-5216
Publisher:Springer (Germany)
Volume:129
Number:6
Page Range:349-354
Date:June 2003
Official Publication:https://doi.org/10.1007/s00432-003-0440-z
PubMed:View item in PubMed

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