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YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression

Item Type:Article
Title:YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression
Creators Name:Juerchott, K. and Bergmann, S. and Stein, U. and Walther, W. and Janz, M. and Manni, I. and Piaggio, G. and Fietze, E. and Dietel, M. and Royer, H.D.
Abstract:Expression of the Y-box protein YB-1 is increased in proliferating normal and cancer cells, but its role in cell proliferation and cell cycle progression is unclear. We have identified a cell cycle-dependent relocalization of YB-1 from the cytoplasm to the nucleus at the G1/S phase transition and demonstrate that both the charged zipper and the cold shock domain are involved in regulating this process. Using cell lines that constitutively overexpress YB-1, we show that nuclear accumulation of YB-1 is associated with increased cyclin A and cyclin B1 mRNA and protein expression. We provide evidence that deregulated YB-1 expression is linked to adhesion-independent cell proliferation through the induction of cyclin A. Thus, we have identified YB-1 as a cell cycle stage-specific transcription factor important for cell proliferation.
Keywords:Amino Acid Motifs, Breast Neoplasms, CCAAT-Enhancer-Binding Proteins, Cell Adhesion, Cell Cycle, Cell Division, Cell Line, Cell Nucleus, Cell Separation, Cyclin A, Cyclin B, Cytoplasm, DNA-Binding Proteins, Flow Cytometry, Fluorescence Microscopy, G1 Phase, Hela Cells, Immunoblotting, Immunohistochemistry, Kinetics, Messenger RNA, Mutation, Neoplastic Gene Expression Regulation, NFI Transcription Factors, Promoter Regions, Reporter Genes, Reverse Transcriptase Polymerase Chain Reaction, S Phase, Tertiary Protein Structure, Transcription Factors, Transfection, Ubcellular Fractions, Y-Box-Binding Protein 1
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:27988-27996
Date:1 January 2003
Official Publication:https://doi.org/10.1074/jbc.M212966200
PubMed:View item in PubMed

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