Cloning, sequencing and recombinant expression of the open reading frame encoding a novel member of the Sarcocystis muris (Apicomplexa) microneme lectin family

Item Type:	Article
Title:	Cloning, sequencing and recombinant expression of the open reading frame encoding a novel member of the Sarcocystis muris (Apicomplexa) microneme lectin family
Creators Name:	Klein, H. and Mueller, S. and Loeschner, B. and Toenjes, R.R. and Braun, G. and Mueller, E.C. and Otto, A. and Montag, T.
Abstract:	Micronemes are characteristic secretory organelles located within the apical cell region of apicomplexan parasites. The protein contents are exocytosed during an early phase of host cell invasion and contribute to parasite motility and the invasion of target cells. We report here on the cloning and heterologous expression of a novel member of the Sarcocystis muris microneme lectin family. The deduced amino acid sequence is in total agreement with that obtained after sequencing the native protein and is characterized by two copies of the apple domain motif. The recombinant polypeptide is expressed in a biologically active conformation as demonstrated by its galactose binding properties.
Keywords:	Amino Acid Sequence, Protozoan Antigens, Baculoviridae, Base Sequence, Cultured Cells, Galectins, Gene Expression Regulation, Protozoan Genes, Molecular Cloning, Molecular Sequence Data, Open Reading Frames, Recombinant Proteins, Sarcocystis, Sequence Alignment, Animals, Insects
Source:	Parasitology Research
ISSN:	0932-0113
Publisher:	Springer
Volume:	90
Number:	1
Page Range:	84-86
Date:	May 2003
Official Publication:	http://link.springer.com/article/10.1007/s00436-002-0600-0
PubMed:	View item in PubMed

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