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Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines

Item Type:Article
Title:Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines
Creators Name:Seitz, S. and Wassmuth, P. and Plaschke, J. and Schackert, H.K. and Karsten, U. and Santibanez-Koref, M.F. and Schlag, P.M. and Scherneck, S.
Abstract:At present, there is conflicting evidence whether microsatellite instability (MSI) plays a role in the pathogenesis of breast cancer. Here we describe for the first time an MSI+ phenotype in two breast cancer cell lines, CAL51 and MT-3, resembling that observed in colorectal cancers. These cell lines are characterized by near-diploid and hyperdiploid karyotypes, respectively. We detected MSI in these cell lines within two non-coding (BAT-25 and BAT-26) and within coding repeat sequences of genes known to be mutated in MSI+ cancer (TGFBR2, IGF2R, BAX). We provide evidence that the inactivation of MMR genes is responsible for MSI in these cell lines.
Keywords:Base Pair Mismatch, Bcl-2-Associated X Protein, Breast Neoplasms, Cultured Tumor Cells, DNA Mutational Analysis, DNA Repair, Gene Amplification, Gene Expression Profiling, IGF Type 2 Receptor, Microsatellite Repeats, Mutation, Neoplasm DNA, Neoplastic Gene Expression Regulation, Nucleic Acid Heteroduplexes, Ovarian Neoplasms, Phenotype, Polyploidy, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Transforming Growth Factor Beta Receptors
Source:Genes Chromosomes & Cancer
ISSN:1045-2257
Publisher:Wiley (U.S.A.)
Volume:37
Number:1
Page Range:29-35
Date:May 2003
Official Publication:https://doi.org/10.1002/gcc.10196
PubMed:View item in PubMed

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