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Detection of microsatellite alterations in the DNA isolated from tumor cells and from plasma DNA of patients with lung cancer

Item Type:Article
Title:Detection of microsatellite alterations in the DNA isolated from tumor cells and from plasma DNA of patients with lung cancer
Creators Name:Bruhn, N. and Beinert, T. and Oehm, C. and Jandrig, B. and Petersen, I. and Chen, X.Q. and Possinger, K. and Fleischhacker, M.
Abstract:In this paper, we show that the same panel of three microsatellite markers is useful for the detection of alterations in the DNA of tumor cells and plasma from patients diagnosed with SCLC and NSCLC. In 31% of the SCLC patients, we detected a microsatellite alteration(s) or LOH in at least one locus. In the group of patients diagnosed with NSCLC, a microsatellite alteration or LOH was detected in at least one locus in 33% of the patients. In all but 2 patients, the identical alteration observed in the DNA from tumor cells was also detected in the DNA isolated from blood plasma. This work confirms the results described by other groups and it extends the diagnostic possibilities of finding tumor cell-specific DNA alterations also in the DNA freely circulating in plasma and serum of patients with cancer.
Keywords:Lung Neoplasms, Microsatellite Repeats, Neoplasm DNA, Non-Small-Cell Lung Carcinoma, Pair 6 Human Chromosomes, Pair 21 Human Chromosomes, Polymerase Chain Reaction, Small Cell Carcinoma, X Chromosome
Source:Annals of the New York Academy of Sciences
ISSN:0077-8923
Publisher:New York Academy of Sciences (U.S.A.)
Volume:906
Page Range:72-82
Date:April 2000
Official Publication:https://doi.org/10.1111/j.1749-6632.2000.tb06594.x
PubMed:View item in PubMed

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