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Assessment of genomic instability in breast cancer and uveal melanoma by random amplified polymorphic DNA analysis

Item Type:Article
Title:Assessment of genomic instability in breast cancer and uveal melanoma by random amplified polymorphic DNA analysis
Creators Name:Papadopoulos, S. and Benter, T. and Anastassiou, G. and Pape, M. and Gerhard, S. and Bornfeld, N. and Ludwig, W.D. and Doerken, B.
Abstract:Some types of cancer have been associated with abnormal DNA fingerprinting. We used random amplified polymorphic DNA (RAPD) to generate fingerprints that detect genomic alterations in human breast cancer. Primers were designed by choosing sequences involved in the development of DNA mutations. Seventeen primers in 44 different combinations were used to screen a total of 6 breast cancer DNA/normal DNA pairs and 6 uveal melanoma DNA/normal DNA pairs. Forty-five percent of these combinations reliably detected quantitative differences in the breast cancer pairs, while only 18% of these combinations detected differences in the uveal melanoma pairs. Fourteen (32%) and 12 (27%) primers generated a smear or did not produce any band patterns in the first and second cases, respectively. Taking into account the ability of RAPD to screen the whole genome, our results suggest that the genomic damage in breast cancer is significantly higher than in uveal melanoma. Our study confirms other reports that the molecular karyotypes produced with random priming, called amplotypes, are very useful for assessing genomic damage in cancer.
Keywords:Genomic Instability, Breast Cancer, Uveal Melanoma, Random Amplified Polymorphic DNA Analysis, Amplotype
Source:International Journal of Cancer
ISSN:0020-7136
Publisher:Wiley (U.S.A.)
Volume:99
Number:2
Page Range:193-200
Date:10 May 2002
Official Publication:https://doi.org/10.1002/ijc.10297
PubMed:View item in PubMed

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