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Expression of B-cell-attracting chemokine 1 (CXCL13) by malignant lymphocytes and vascular endothelium in primary central nervous system lymphoma

Official URL:https://doi.org/10.1182/blood-2002-05-1576
PubMed:View item in PubMed
Creators Name:Smith, J.R. and Braziel, R.M. and Paoletti, S. and Lipp, M. and Uguccioni, M. and Rosenbaum, J.T.
Journal Title:Blood
Journal Abbreviation:Blood
Volume:101
Number:3
Page Range:815-821
Date:1 February 2003
Keywords:Brain, Case-Control Studies, CC Chemokines, Central Nervous System Neoplasms, Chemokine Receptors, CXC Chemokines, Cytokine Receptors, Immunohistochemistry, Lymphocytes, Lymphoma, Messenger RNA
Abstract:Primary central nervous system lymphoma (PCNSL) is a rare but often rapidly fatal form of non-Hodgkin B-cell lymphoma that arises within the central nervous system (CNS) and has a low propensity to metastasize. We performed immunohistochemistry on formalin-fixed, paraffin-embedded brain biopsy specimens from 24 patients with PCNSL to investigate the expression of B cell-attracting chemokine 1 (BCA-1, CXCL13), a lymphoid chemokine involved in B-cell compartmental homing within secondary lymphoid organs and recently implicated in the pathogenesis of inflammatory and malignant lymphocyte-mediated diseases. Whereas BCA-1 was not detected in normal human brain, all 24 brain biopsy specimens containing PCNSL were positive for BCA-1. Double immunostaining on selected specimens localized BCA-1 to malignant B lymphocytes and vascular endothelium. In contrast, 2 chemokines implicated particularly in T-cell movement, secondary lymphoid tissue chemokine (SLC, CCL21) and Epstein-Barr virus-induced molecule 1 ligand chemokine (ELC, CCL19), were expressed only by occasional stromal cells in 2 and 4 of the 24 specimens, respectively. Tumor cells stained positively for CXCR5, the primary receptor for BCA-1. In situ hybridization verified the expression of BCA-1 mRNA by malignant B cells, but not vascular endothelium, within the tumor mass, suggesting that vascular endothelial BCA-1 expression may be consequent to transcytosis. In PCNSL, expression of BCA-1 by malignant lymphocytes and vascular endothelium may influence tumor development and localization to CNS.
ISSN:0006-4971
Publisher:American Society of Hematology (U.S.A.)
Item Type:Article

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