Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen: Negative effect of disaccharides in vitrification solution

Item Type:	Article
Title:	Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen: Negative effect of disaccharides in vitrification solution
Creators Name:	Isachenko, V. and Isachenko, E. and Rahimi, G. and Krivokharchenko, A. and Alabart, J.L. and Nawroth, F.
Abstract:	Given that it has been possible to successfully cryopreserve human ovarian tissue by direct plunging into liquid nitrogen, this study was designed to establish the future direction to be taken in this line of research. Bovine oviductal epithelial fragments (as a tissue model) and large biopsy fragments (~2.0 mm3) of human ovarian tissue were used for cryopreservation. Two protocols were tested: with permeable cryoprotectants (dimethyl sulphoxide, propylene glycol, glycerol) + egg yolk + sucrose or trehalose + a synthetic blocker of ice nucleation, Supercool® X-1000; and using only permeable cryoprotectants (glycerol and ethylene glycol) + egg yolk + Supercool® X-1000. The cryopreserved tissue specimens were subsequently thawed and the cryoprotectants removed by dilution in graded sucrose solutions. Both the dynamic growth and hormonal activity of the ovarian tissue pieces, vitrified using only permeable cryoprotectants, were greater than after vitrification in a mixture of permeable cryoprotectants and sucrose. Unlike the case for other reproductive tissue (spermatozoa, oocytes, embryos), these findings suggest that the cryopreservation of ovarian tissue by direct plunging into liquid nitrogen must be achieved by vitrification using only permeable cryoprotectants and agents that prevention ice formation.
Keywords:	Human Ovarian Tissue Without Follicles, Vitrification, Development, Estradiol-17{beta}, Progesterone, Animals, Cattle
Source:	Cryoletters
ISSN:	0143-2044
Volume:	23
Number:	5
Page Range:	333-344
Date:	1 September 2002
Official Publication:	http://www.ingentaconnect.com/contentone/cryo/cryo/2002/00000023/00000005/art00008
PubMed:	View item in PubMed

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