Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Counting CAG repeats in the Huntington's disease gene by restriction endonuclease EcoP15I cleavage

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
257kB

Item Type:Article
Title:Counting CAG repeats in the Huntington's disease gene by restriction endonuclease EcoP15I cleavage
Creators Name:Moencke-Buchner, E. and Reich, S. and Muecke, M. and Reuter, M. and Messer, W. and Wanker, E.E. and Krueger, D.H.
Abstract:Huntington's disease (HD) is a progressive neurodegenerative disorder with autosomal-dominant inheritance. The disease is caused by a CAG trinucleotide repeat expansion located in the first exon of the HD gene. The CAG repeat is highly polymorphic and varies from 6 to 37 repeats on chromosomes of unaffected individuals and from more than 30 to 180 repeats on chromosomes of HD patients. In this study, we show that the number of CAG repeats in the HD gene can be determined by restriction of the DNA with the endonuclease EcoP15I and subsequent analysis of the restriction fragment pattern by electrophoresis through non-denaturing polyacrylamide gels using the ALFexpress DNA Analysis System. CAG repeat numbers in the normal (30 and 35 repeats) as well as in the pathological range (81 repeats) could be accurately counted using this assay. Our results suggest that this high-resolution method can be used for the exact length determination of CAG repeats in HD genes as well as in genes affected in related CAG repeat disorders.
Keywords:DNA Mutational Analysis, Type III Site-Specific Deoxyribonucleases, Exons, Huntington Disease, Molecular Sequence Data, Nerve Tissue Proteins, Nuclear Proteins, Restriction Fragment Length Polymorphism, Proteins, Trinucleotide Repeat Expansion
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press (U.K.)
Volume:30
Number:16
Page Range:e83
Date:15 August 2002
Official Publication:https://doi.org/10.1093/nar/gnf082
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library