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CD4+CD3- cells induce Peyer's patch development: role of {alpha}4 {beta}1 integrin activation by CXCR5

Official URL:https://doi.org/10.1016/S1074-7613(02)00395-3
PubMed:View item in PubMed
Creators Name:Finke, D. and Acha-Orbea, H. and Mattis, A. and Lipp, M. and Kraehenbuhl, J.P.
Journal Title:Immunity
Journal Abbreviation:Immunity
Page Range:363-373
Date:September 2002
Keywords:Adoptive Transfer, CD3 Antigens, CD4-Positive T-Lymphocytes, Cell Adhesion, CXC Chemokines, Immunologic Deficiency Syndromes, Integrin alpha4beta1, Inbred C57BL Mice, Immunological Models, Knockout Mice, Morphogenesis, Peyers Patches, Radiation Chimera, Chemokine Receptors, Cytokine Receptors, Signal Transduction, Stromal Cells, Vascular Cell Adhesion Molecule-1, Animals, Mice
Abstract:CD4+CD3- cells are the predominant hematopoietic cells found in mouse fetal intestine. We prove their role as Peyers patch (PP)-inducing cells by transfer into neonatal PP-deficient mice. To test the requirement of chemokines and adhesion molecules in induction of PP, we studied mice deficient in CXCR5 and/or {alpha}4{beta}1 integrin-mediated adhesion. CXCR5-/- mice have CD4+CD3- cells, which are inefficient in inducing PP formation. We show here that CXCR5/CXCL13 signaling activates {alpha}4{beta}1 integrin on CD4+CD3- cells. Blocking of {beta}1 integrin or VCAM-1, the ligand of {alpha}4{beta}1 integrin, inhibits PP formation. This study demonstrates the link between chemokine receptors and adhesion molecules that regulates stromal/hematopoietic cell interaction leading to PP formation.
Publisher:Cell Press (U.S.A.)
Item Type:Article

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