Tumor growth inhibition elicited by different vaccines and correlation with antigen specific cytotoxic T-cell frequencies determined by intracellular interferon-γ staining

Item Type:	Article
Title:	Tumor growth inhibition elicited by different vaccines and correlation with antigen specific cytotoxic T-cell frequencies determined by intracellular interferon-γ staining
Creators Name:	Johnen, H. and Pecher, G.
Abstract:	Different vaccines based on naked DNA and the modified vaccinia virus Ankara (MVA) were compared for their efficiency to protect mice against tumors bearing the model antigen beta-galactosidase ({beta}-Gal) and for their potential to induce an antigen specific cellular immune response. Mice were immunized with the LacZ gene applied as naked DNA. In accordance with the observed {beta}-Gal-specific T-cell frequency, only 20% of mice boosted with LacZ naked DNA developed tumors whereas all mice boosted with MVA expressing LacZ developed a tumor. Mice vaccinated with mock DNA or mock virus developed tumors in 60 or 100%, respectively. MVA vaccination led to strong and long-lasting CD4- and CD8-T-cell responses against viral antigens but not against {beta}-Gal.
Keywords:	Naked DNA, Modified Vaccinia Virus Ankara, Vaccine, Intracellular Interferon-{gamma} Staining, Mouse Tumor Model, Animals, Mice
Source:	Cancer Letters
ISSN:	0304-3835
Volume:	185
Number:	1
Page Range:	53-59
Date:	8 November 2002
Official Publication:	https://doi.org/10.1016/S0304-3835(02)00321-X
PubMed:	View item in PubMed

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