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GABA- and glutamate-activated currents in glial cells of the mouse corpus callosum slice

Item Type:Article
Title:GABA- and glutamate-activated currents in glial cells of the mouse corpus callosum slice
Creators Name:Berger, T. and Walz, W. and Schnitzer-Ungefug, J. and Kettenmann, H.
Abstract:Whole-cell transmitter-activated currents were recorded with the patch-clamp technique from glial cells in thin frontal brain slices of the corpus callosum. In slices from 6- to 8-day-old mice, glioblasts were predominantly found, while oligodendrocytes were predominant in slices from 10- to 13-day-old mice. These developmental stages could be readily distinguished by their K+ channel pattern and their morphology and ultrastructural features. Both cell types expressed GABA and glutamate receptors in this in situ preparation. GABA responses showed similarities to those described for GABAA receptors, i.e., they were mimicked by muscimol, blocked by bicuculline, and enhanced by pentobarbital. Glutamate responses showed similarities to those of the kainate/quisqualate receptor subtype. The amplitude of GABA-activated currents recorded in oligodendrocytes was significantly smaller than that from glioblasts, while glutamate responses did not show marked differences in either cell type.
Keywords:Action Potentials, Bicuculline, Chloride Channels, Corpus Callosum, Glutamates, Glutamic Acid, Ion Channel Gating, Kainic Acid, Membrane Proteins, Muscimol, Oligodendroglia, Pentobarbital, Potassium Channels, GABA-A Receptors, Glutamate Receptors, Neurotransmitter Receptors, Stem Cells, Gamma-Aminobutyric Acid, Animals, Mice
Source:Journal of Neuroscience Research
ISSN:0360-4012
Publisher:Wiley (U.S.A.)
Volume:31
Number:1
Page Range:21-27
Date:1 January 1992
Official Publication:https://doi.org/10.1002/jnr.490310104
PubMed:View item in PubMed

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