An immunodominant, cross-reactive B-cell epitope region is located at the C-terminal part of the hamster polyomavirus major capsid protein VP1

Item Type:	Article
Title:	An immunodominant, cross-reactive B-cell epitope region is located at the C-terminal part of the hamster polyomavirus major capsid protein VP1
Creators Name:	Siray, H. and Frommel, C. and Voronkova, T. and Hahn, S. and Arnold, W. and Schneider-Mergener, J. and Scherneck, S. and Ulrich, R.
Abstract:	The VP1 represents the major capsid protein of the hamster polyomavirus (HaPV). Here we describe the mapping of epitopes along the VP1 using Escherichia coli-expressed VP1-dihydrofolate reductase (DHFR) fusion proteins and PepScan analysis. By use of DHFR fusion proteins an immunodominant region was localized in the C-terminal part of VP1 between amino acids 320-384. Further epitopes are located in the regions amino acids 1-133 and amino acids 133-320, respectively. There were no obvious differences in the reactivity between sera of tumor-bearing and papilloma-free naturally HaPV-infected hamsters. In contrast, PepScan analysis revealed linear epitopes in the regions amino acids 79-97 and amino acids 353-367 for tumor-bearing animals and amino acids 101-113 and amino acids 165-179 for papilloma-free animals. The region between amino acids 320-384 of HaPV-VP1 was found to be involved in cross-reactivity of VP1 from HaPV and other polyomaviruses. Previously we have demonstrated that heterologous expression of HaPV-VP1 allowed the formation of virus-like particles (VLPs). From epitope mapping data and structural predictions it has been suggested that HaPV-VP1-VLPs may tolerate foreign peptides in the region amino acids 81-88 and the C-terminal part of VP1.
Keywords:	Amino Acid Sequence, Capsid, Capsid Proteins, Cricetinae, Cross Reactions, Epitope Mapping, B-Lymphocyte Epitopes, Immune Sera, Immunodominant Epitopes, Polyomavirus, Polyomavirus Infections, Recombinant Fusion Proteins, Tumor Virus Infections, Viral Antibodies, Animals, Papilloma
Source:	Viral Immunology
ISSN:	0882-8245
Publisher:	Mary Ann Liebert
Volume:	13
Number:	4
Page Range:	533-545
Date:	2000
Official Publication:	https://doi.org/10.1089/vim.2000.13.533
PubMed:	View item in PubMed

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