Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

Item Type:	Article
Title:	Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
Creators Name:	Gonczy, P. and Echeverri, G. and Oegema, K. and Coulson, A. and Jones, S.J. and Copley, R.R. and Duperon, J. and Oegema, J. and Brehm, M. and Cassin, E. and Hannak, E. and Kirkham, M. and Pichler, S. and Flohrs, K. and Goessen, A. and Leidel, S. and Alleaume, A.M. and Martin, C. and Ozlu, N. and Bork, P. and Hyman, A.A.
Abstract:	Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of approximately 96% of the approximately 2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (approximately 6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that approximately 47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.
Keywords:	Caenorhabditis Elegans, Cell Division, Chromosomes, Helminth Genes, Genomics, Open Reading Frames, Helminth RNA
Source:	Nature
ISSN:	0028-0836
Publisher:	Nature Publishing Group
Volume:	408
Number:	6810
Page Range:	331-336
Date:	16 November 2000
Official Publication:	https://doi.org/10.1038/35042526
PubMed:	View item in PubMed

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