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Evidence for a NOD2-independent susceptibility locus for inflammatory bowel disease on chromosome 16p

Official URL:https://doi.org/10.1073/pnas.261567999
PubMed:View item in PubMed
Creators Name:Hampe, J. and Frenzel, H. and Mirza, M.M. and Croucher, P.J. and Cuthbert, A. and Mascheretti, S. and Huse, K. and Platzer, M. and Bridger, S. and Meyer, B. and Nuernberg, P. and Stokkers, P. and Krawczak, M. and Mathew, C.G. and Curran, M. and Schreiber, S.
Journal Title:Proceedings of the National Academy of Sciences of the United States of America
Journal Abbreviation:Proc Natl Acad Sci U S A
Volume:99
Number:1
Page Range:321-326
Date:8 January 2002
Keywords:Carrier Proteins, DNA Mutational Analysis, Family Health, Genetic Predisposition to Disease, Genotype, Haplotypes, Human Pair 16 Chromosomes, Inflammatory Bowel Diseases, Intracellular Signaling Peptides and Proteins, Linkage (Genetics), Lod Score, Microsatellite Repeats, Nod2 Signaling Adaptor Protein, Phenotype, Single Nucleotide Polymorphism
Abstract:Heritable predisposition to inflammatory bowel disease (IBD) has been demonstrated by epidemiological and genetic analysis. Linkage of IBD to broad regions of chromosome 16 has been established by analysis of multiple populations. NOD2, located on proximal 16q, was recently identified as an IBD gene. As the linkage regions on chromosome 16 are large, we have investigated the possibility that NOD2 is not the only IBD gene located on this chromosome. A high-density experiment using 39 microsatellite markers was performed to identify additional regions of association, and to indicate areas of interest for further investigation. A triple-peaked configuration of the linkage curve with peak logarithm of odds (Iod) scores of 2.7, 3.2, and 3.1 was observed on proximal 16p, proximal 16q, and central 16q, respectively. The cohort was stratified by coding individuals carrying the NOD2 single nucleotide polymorphism (SNP)8 and SNP13 "unknown." Significance at the central peak, corresponding to the genomic location of NOD2, then decreased from 3.2 to 1.2. The maximal Iod scores on the proximal p-arm (lod = 2.1) and central q-arm (Iod = 2.6) changed only moderately. An exploratory association analysis (TRANSMIT) yielded a strong lead at D1653068 (P = 0.00028). The region around this marker was further investigated by using anonymous SNPs. An associated haplotype containing three SNPs was identified (peak significance P = 0.00027, IBD phenotype). On stratification based on NOD2 genotype, this significance increased to P = 0.0001. These results confirm the importance of NOD2 and provide evidence for a second IBD gene located on chromosome 16p.
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Item Type:Article

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