Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Smoothelin contains a novel actin cytoskeleton localization sequence with similarity to troponin T

Item Type:Article
Title:Smoothelin contains a novel actin cytoskeleton localization sequence with similarity to troponin T
Creators Name:Quensel, C. and Kraemer, J. and Cardoso, M.C. and Leonhardt, H.
Abstract:Smoothelin, a cytoskeletal protein, exists in a large isoform specifically expressed in vascular smooth muscle cells, and a small visceral isoform generated by a downstream transcriptional start site. Using fusions to the green fluorescent protein, we could show that both smoothelin isoforms are localized at actin containing filaments and mapped two domains that are each sufficient for localization at the actin cytoskeleton. The first domain is located in the vascular-specific, N-terminal half of smoothelin and the second in the common, C-terminal half. The second domain shares clear sequence similarity with a domain of troponin T involved in actin filament association. These results suggest that the tissue-specific expression of smoothelin isoforms might contribute to the different contractile properties of smooth muscle cells.
Keywords:Actin, Smoothelin, Troponin T, Cytoskeleton, Muscle Contraction, Smooth Muscle, Animals, Chickens, Mice
Source:Journal of Cellular Biochemistry
ISSN:0730-2312
Publisher:Wiley (U.S.A.)
Volume:85
Number:2
Page Range:403-409
Date:2002
Official Publication:https://doi.org/10.1002/jcb.10143
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library