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Increased tumorigenicity, but unchanged immunogenicity, of transporter for antigen presentation 1-deficient tumors

Item Type:Article
Title:Increased tumorigenicity, but unchanged immunogenicity, of transporter for antigen presentation 1-deficient tumors
Creators Name:Qin, Z. and Harders, C. and Cao, X. and Huber, C. and Blankenstein, T. and Seliger, B.
Abstract:The lack of transporter-for-antigen-presentation (TAP)-1 expression by tumor cells prevents the processing and presentation of MHC class I-restricted tumor antigens. This could affect T-cell-dependent tumor immunity in either the priming or the effector phase. We have established TAP1+ and TAP1− tumor cell lines using ras-transformed NIH3T3 fibroblasts. Impaired TAP1 expression by tumor cells increased their tumorigenicity in immunocompetent, but not in T-cell-deficient, mice. For the generation of tumor immunity, TAP1 expression was not necessary on tumor cells used for vaccination. However, in previously immunized mice TAP1+ tumor cells were more efficiently rejected than were TAP1− tumor cells. CD8+ T cells infiltrated both TAP1+- and TAP1−-challenge tumors and were required for tumor rejection. In mixed tumor/lymphocyte culture, TAP1 expression by tumor cells significantly increased the IFN-γ production of antigen-specific spleen cells from immunized, but not from naive, mice. Thus, the lack of TAP1 expression did not change the immunogenicity of tumor cells. It may enable tumor cells to escape T-cell recognition during the effector phase of an antitumor immune response.
Keywords:3T3 Cells, ATP-Binding Cassette Transporters, Antigen Presentation, CD8-Positive T-Lymphocytes, Experimental Neoplasms, Inbred BALB C Mice, Inbred DBA Mice, Interferon-{gamma}, Nude Mice, Spleen, T-Lymphocytes, Transfection, Transformed Cell Line, Tumor-Infiltrating Lymphocytes, Animals, Mice
Source:Cancer Research
Publisher:American Association for Cancer Research
Page Range:2856-2860
Date:15 May 2002
Official Publication:http://cancerres.aacrjournals.org/cgi/content/abstract/62/10/2856
PubMed:View item in PubMed

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