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Structure of transfection-active histone H1/DNA complexes

Item Type:Article
Title:Structure of transfection-active histone H1/DNA complexes
Creators Name:Lucius, H. and Haberland, A. and Zaitsev, S. and Dalluege, R. and Schneider, M. and Boettger, M.
Abstract:Relationships between the structure of transfecting complexes of histone H1 and DNA and their transfection efficiency were studied. Transfection activity proved to be connected to complex aggregates. Low speed centrifugation of the complexes resulted in loss of the transfection activity. The complexes/aggregates were active with high efficiency in a broad range of weight input ratios r(i) (0.1 < r(i) < 30). Using atomic force microscopy (AFM), the complexes were imaged at negative, nearly electroneutral and positive charge conditions. Electroneutral complexes at r(i) = 1 showed a multitude of different complex forms. Fibrillar, network-like and branched structures were frequently present in one complex. Strongly positive charged complexes had a toroidal appearance. All these different forms contributed to the high transfection efficiency. Cellular uptake is supposed to be by phagocytosis.
Keywords:Aggregation, Atomic Force Microscopy, H1 Histone, H1/DNA Complexes, Transfection, Animals, Cattle
Source:Molecular Biology Reports
ISSN:0301-4851
Publisher:Kluwer Academic Publ (The Netherlands)
Volume:28
Number:3
Page Range:157-165
Date:September 2002
Official Publication:https://doi.org/10.1023/A:1015230010230
PubMed:View item in PubMed

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