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Cell surface α2,6-sialylation affects adhesion of breast carcinoma cells

Item Type:Article
Title:Cell surface α2,6-sialylation affects adhesion of breast carcinoma cells
Creators Name:Lin, S.Q. and Kemmner, W. and Grigull, S. and Schlag, P.M.
Abstract:Tumor-associated alterations of cell surface glycosylation play a crucial role in the adhesion and metastasis of carcinoma cells. The aim of this study was to examine the effect of {alpha}2,6-sialylation on the adhesion properties of breast carcinoma cells. To this end mammary carcinoma cells, MDA-MB-435, were sense-transfected with sialyltransferase ST6Gal-I cDNA or antisense-transfected with a part of the ST6Gal-I sequence. Sense transfectants showed an enhanced ST6Gal-I mRNA expression and enzyme activity and an increased binding of the lectin Sambucus nigra agglutinin (SNA), specific for {alpha}2,6-linked sialic acid. Transfection with ST6Gal-I in the antisense direction resulted in less enzyme activity and SNA reactivity. A sense-transfected clone carrying increased amounts of {alpha}2,6-linked sialic acid adhered preferentially to collagen IV and showed reduced cell-cell adhesion and enhanced invasion capacity. In contrast, antisense transfection led to less collagen IV adhesion but enhanced homotypic cell-cell adhesion. In another approach, inhibition of ST6Gal-I enzyme activity by application of soluble antisense-oligodeoxynucleotides was studied. Antisense treatment resulted in reduced ST6 mRNA expression and cell surface 2,6-sialylation and significantly decreased collagen IV adhesion. Our results suggest that cell surface {alpha}2,6-sialylation contributes to cell-cell and cell-extracellular matrix adhesion of tumor cells. Inhibition of sialytransferase ST6Gal-I by antisense-oligodeoxynucleotides might be a way to reduce the metastatic capacity of carcinoma cells.
Keywords:Antisense, Cell Adhesion, Mammary Neoplasm, Neoplasm Metastasis, Sialyltransferases
Source:Experimental Cell Research
ISSN:0014-4827
Publisher:Academic Press (U.S.A.)
Volume:276
Number:1
Page Range:101-110
Date:15 May 2002
Official Publication:https://doi.org/10.1006/excr.2002.5521
PubMed:View item in PubMed

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