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Characterization of structure and mechanism of transfection-active peptide-DNA complexes

Item Type:Article
Title:Characterization of structure and mechanism of transfection-active peptide-DNA complexes
Creators Name:Dalluege, R. and Haberland, A. and Zaitsev, S. and Schneider, M. and Zastrow, H. and Sukhorukov, G. and Boettger, M.
Abstract:We studied a number of physicochemical parameters of transfection-active peptide-DNA complexes including size, aggregation behaviour and circular dichroism (CD) spectra. These data were brought in relationship to the transfection activity of these peptides in order to better understand the mechanism of peptide-mediated gene transfer. A DNA binding oligolysine (K 16) and a peptide comprising K 16 with an added peptide loop containing the arbitrary sequence RAD not known as a receptor ligand were used. Whereas the K 16-DNA complex at 88% charge neutralization of the DNA phosphates collapsed into small toroidal particles with a diameter of 200 nm by dynamic light scattering, K 16-cRAD did not. Instead, large aggregates were observed. CD spectra showed that the K 16-DNA complexes were in a -{psi} state observed at liquid crystalline phases. Increasing positive charge by addition of further K 16 or disturbing the -{psi} state by introducing the RAD-peptide loop resulted in increasing instability indicated by aggregation and loss of the -{psi} CD spectrum of the complexes. Transfection experiments indicated that the aggregated material was the transfection-active component.
Keywords:Transfection, Peptide-DNA Complex, Aggregation, Dynamic Light Scattering, Atomic Force Microscopy, Circular Dichroism Spectroscopy
Source:Biochimica et Biophysica Acta - Gene Structure and Expression
Page Range:45-52
Date:7 June 2002
Official Publication:https://doi.org/10.1016/S0167-4781(02)00291-9
PubMed:View item in PubMed

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