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Roles of SLC/CCL21 and CCR7 in human kidney for mesangial proliferation, migration, apoptosis, and tissue homeostasis

Item Type:Article
Title:Roles of SLC/CCL21 and CCR7 in human kidney for mesangial proliferation, migration, apoptosis, and tissue homeostasis
Creators Name:Banas, B. and Woernle, M. and Berger, T. and Nelson, P.J. and Cohen, C.D. and Kretzler, M. and Pfirstinger, J. and Mack, M. and Lipp, M. and Groene, H.J. and Schloendorff, D.
Abstract:The release of chemokines by intrinsic renal cells is an important mechanism for the regulation of leukocyte trafficking during renal inflammation. The expression of chemokine receptors by intrinsic renal cells such as mesangial cells (MC) suggests an expanded role for chemokine-chemokine receptor biology in local immunomodulation and potentially glomerular homeostasis. By immunohistochemistry we found the chemokine receptor CCR7 expressed in a mesangial pattern while the CCR7 ligand SLC/CCL21 showed a podocyte-specific expression. CCR7 expression was further characterized by RT-PCR, RNase protection assays, and FACS analysis of cultured human MC, and was found to be constitutively present. Real-time PCR of microdissected glomeruli confirmed the expression of SLC/CCL21. A functional role for CCR7 was demonstrated for human MC migration and proliferation. A protective effect of SLC/CCL21 was shown for MC survival in Fas Ab-induced apoptosis. Finally, "wound healing" was enhanced in the presence of SLC/CCL21 in an in vitro injury model. The constitutive glomerular expression of CCR7 and its ligand SLC/CCL21 in adjacent cell types of the human kidney suggests novel biological functions of this chemokine/chemokine receptor pair and a potential role in processes involved in glomerular homeostasis and regeneration.
Keywords:Apoptosis, CC Chemokines, CCR7 Receptors, Cell Division, Cell Survival, Chemokine CCL21, Chemokine Receptors, Chemotaxis, Glomerular Mesangium, Homeostasis, Kidney, Kinetics, Messenger RNA, Signal Transduction, Transformed Cell Line
Source:Journal of Immunology
ISSN:0022-1767
Publisher:American Association of Immunologists (U.S.A.)
Volume:168
Number:9
Page Range:4301-4307
Date:1 May 2002
Official Publication:http://www.jimmunol.org/content/168/9/4301.long#fn-2
PubMed:View item in PubMed

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