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Loss of PU.1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease

Item Type:Article
Title:Loss of PU.1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease
Creators Name:Jundt, F. and Kley, K. and Anagnostopoulos, I. and Schulze-Proebsting, K. and Greiner, A. and Mathas, S. and Scheidereit, C. and Wirth, T. and Stein, H. and Doerken, B.
Abstract:Immunoglobulin transcription is impaired in Hodgkin and Reed-Sternberg (HRS) cells of classical Hodgkin disease (cHD). We recently demonstrated that defective immunoglobulin promoter transcription correlates with the down-regulation of the B-cell transcription factors Oct2 and BOB.1/OBF.1. These results prompted us to investigate whether immunoglobulin enhancer activity is also impaired in HRS cells and whether as yet unidentified factors could be necessary for immunoglobulin enhancer activity in HRS cells of cHD. Here we analyzed 30 cases of cHD for expression of the Ets family member PU.1 that is known to collaborate with multiple transcription factors and to regulate expression of immunoglobulin genes. We show that PU.1 is not expressed in primary and cultured HRS cells. Reintroduction of PU.1 and Oct2 in cultured HRS cells restored the activity of cotransduced immunoglobulin enhancer constructs. Our study identifies PU.1 deficiency as a recurrent defect in HRS cells that might contribute to their impairment of immunoglobulin transcription.
Keywords:Cultured Tumor Cells, DNA-Binding Proteins, Down-Regulation, Genetic Promoter Regions, Genetic Transcription, Hodgkin Disease, Immunoglobulin Genes, Immunoglobulins, Lymph Nodes, Octamer Transcription Factor-2, Proto-Oncogene Proteins, Reed-Sternberg Cells, Trans-Activators, Transcription Factors, Transfection
Source:Blood
ISSN:0006-4971
Publisher:American Society of Hematology (U.S.A.)
Volume:99
Number:8
Page Range:3060-3062
Date:15 April 2002
Official Publication:https://doi.org/10.1182/blood.V99.8.3060
PubMed:View item in PubMed

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