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Polycation-mediated transfection: How to overcome undesirable side effects of sticky DNA complexes

Item Type:Article
Title:Polycation-mediated transfection: How to overcome undesirable side effects of sticky DNA complexes
Creators Name:Haberland, A. and Dalluege, R. and Erdmann, B. and Zaitsev, S. and Cartier, R. and Schaefer-Korting, M. and Boettger, M.
Abstract:Using polycationic transfection one encounters undesired persistent binding to cells of sticky polycation/DNA complexes. These complexes simulate transfection under conditions where no uptake is expected e.g. at 4°C if the uptake is by endocytosis. To overcome this problem, using H1/DNA complexes, we developed an easy and nontoxic method for removing the sticky complexes not taken up during the transfection phase. The cells are simply washed with isotonic (0.1 M) MgCl2 solution, which enables the complete removal of the complexes by their rapid dissolution.
Keywords:Transfection, H1/DNA Complexes, Stickiness, Cell Washing
Source:Somatic Cell and Molecular Genetics
ISSN:0740-7750
Publisher:Plenum Publishing Corporation (U.S.A.)
Volume:25
Number:5-6
Page Range:327-332
Date:November 1999
Official Publication:https://doi.org/10.1023/A:1019920516714
PubMed:View item in PubMed

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