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Experience-induced neurogenesis in the senescent dentate gyrus

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Item Type:Article
Title:Experience-induced neurogenesis in the senescent dentate gyrus
Creators Name:Kempermann, G. and Kuhn, H.G. and Gage, F.H.
Abstract:We demonstrate here that under physiological conditions neurogenesis continues to occur in the dentate gyrus of senescent mice and can be stimulated by living in an enriched environment. Neurogenesis was investigated by confocal microscopy of three-channel immunofluorescent staining for the proliferation marker bromodeoxyuridine (BrdU) and neuronal and glial markers. Quantification was performed with unbiased stereological counting techniques. Neurogenesis decreased with increasing age. Stimulation of adult and aged mice by switching from standard housing to an enriched environment with opportunities for social interaction, exploration, and physical activity for 68 d resulted in an increased survival of labeled cells. Phenotypic analysis revealed that, in enriched living animals, relatively more cells differentiated into neurons, resulting in a threefold net increase of BrdU-labeled neurons in 20-month-old mice (105 vs 32 cells) and a more than twofold increase in 8-month-old mice (684 vs 285 cells) compared with littermates living under standard laboratory conditions. Corresponding absolute numbers of BrdU-positive astrocytes and BrdU-positive cells that did not show colabeling for neuronal or glial markers were not influenced. The effect on the relative distribution of phenotypes can be interpreted as a survival-promoting effect that is selective for neurons. Proliferation of progenitor cells appeared unaffected by environmental stimulation.
Keywords:Aging, Brain, Hippocampus, Neurogenesis, Stem Cell, Progenitor Cell, Enriched Environment, Plasticity, Stereology, Animals, Mice
Source:Journal of Neuroscience
ISSN:0270-6474
Publisher:Society for Neuroscience (U.S.A.)
Volume:18
Number:9
Page Range:3206-3212
Date:1 May 1998
Additional Information:Copyright (c) 1998 by The Society for Neuroscience
Official Publication:http://www.jneurosci.org/cgi/content/abstract/18/9/3206
PubMed:View item in PubMed

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