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Nitric oxide signals parallel fiber activity to Bergmann glial cells in the mouse cerebellar slice

Item Type:Article
Title:Nitric oxide signals parallel fiber activity to Bergmann glial cells in the mouse cerebellar slice
Creators Name:Matyash, V. and Filippov, V. and Mohrhagen, K. and Kettenmann, H.
Abstract:Stimulation of parallel fibers in the cerebellar cortex triggers a transient calcium increase in Bergmann glial cells, a special form of astrocytes. Using patch-clamping and imaging techniques we have found that this form of neuron-gila interaction is mediated by nitric oxide (NO) since the response is blocked by the NO-synthase inhibitor Nω-nitro-L-arginine and mimicked by NO donors. None of the neurotransmitter receptors of Bergmann gila identified so far participates in or interferes with this signaling cascade. The NO-triggered increases in [Ca2+]i, as studied in Bergmann glial cells in the slice or in cultured astrocytes, are due to Ca2+ influx and not to release from cytoplasmic stores. Thus, NO released from parallel fibers serves as a signaling substance to the neighboring glial elements.
Keywords:Action Potentials, Axons, Calcium, Calcium Signaling, Cell Communication, Cerebellar Cortex, Cultured Cells, Cytoplasm, Electric Stimulation, Enzyme Inhibitors, Fluorescent Dyes, Fura-2, Inbred Strains Mice, Neuroglia, Neurotransmitter Receptors, Nitric Oxide, Nitric Oxide Donors, Nitric Oxide Synthase, Organ Culture Techniques, Animals, Mice
Source:Molecular and Cellular Neuroscience
ISSN:1044-7431
Publisher:Academic Press (U.S.A.)
Volume:18
Number:6
Page Range:664-670
Date:December 2001
Official Publication:https://doi.org/10.1006/mcne.2001.1047
PubMed:View item in PubMed

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