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Development and tissue-specific distribution of mouse small heat shock protein hsp25

Item Type:Article
Title:Development and tissue-specific distribution of mouse small heat shock protein hsp25
Creators Name:Gernold, M. and Knauf, U. and Gaestel, M. and Stahl, J. and Kloetzel, P.M.
Abstract:We have investigated the developmental and tissue-specific distribution of the mouse small hsp25 by immunohistology using an antibody that specifically identifies hsp25. Our analysis shows that the relative amount of hsp25 increases during embryogenesis. Through days 13-20 of embryogenesis, hsp25 accumulation is predominant in the various muscle tissues, including the heart, the bladder, and the back muscles. hsp25 is detectable also in neurons of the spinal cord and the purkinje cells. Furthermore analysis of the closely related alpha, B-crystallin shows that in several tissues, including the bladder, the notochordal sheath and the eye lens both proteins are coexpressed. Our studies demonstrate that mammalian hsp25 accumulation is developmentally regulated during mouse embryogenesis and support the view of an important functional role of small heat shock proteins in normal cell metabolism.
Keywords:Antibody Specificity, Cell Differentiation, Crystallins, Embryonic and Fetal Development, Gene Expression Regulation, Heat-Shock Proteins, Immunohistochemistry, Crystalline Lens, Muscles, Nerve Tissue, Organ Specificity, Tissue Distribution, Urinary Bladder, Animals, Mice
Source:Developmental Genetics
Page Range:103-111
Official Publication:https://doi.org/10.1002/dvg.1020140204
PubMed:View item in PubMed

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