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Pharmaceutical evaluation of gas-filled microparticles as gene delivery system

Item Type:Article
Title:Pharmaceutical evaluation of gas-filled microparticles as gene delivery system
Creators Name:Seemann, S. and Hauff, P. and Schultze-Mosgau, M. and Lehmann, C. and Reszka, R.
Abstract:Purpose. To produce and characterize a nonviral ultrasound-controlled release system of plasmid DNA (pDNA) encapsulated in gas-filled poly(D,L-lactide-co-glycolide) microparticles (PLGA-MPs). Methods. Different cationic polymers were used to form pDNA/polymer complexes to enhance the stability of pDNA during microparticle preparation. The physico-acoustical properties of the microparticles, particle size, pDNA integrity, encapsulation efficiency and pDNA release behavior were studied in vitro. Results. The microparticles had an average particle size of around 5 μm. More than 50% of all microparticles contained a gas core, and when exposed to pulsed ultrasound as used for color Doppler imaging create a signal that yields typical color patterns (stimulated acoustic emission) as a result of the ultrasound-induced destruction of the microparticles. Thirty percent of the pDNA used was successfully encapsulated and approximately 10% of the encapsulated pDNA was released by ultrasound within 10 min. Conclusions. Plasmid DNA can be encapsulated in biodegradable gas-filled PLGA-MPs without hints for a structural disintegration. A pDNA release by ultrasound-induced microparticle-destruction could be shown in vitro.
Keywords:Controlled Release, DNA, Gene Therapy, Microparticles, Poly(D,L-lactide-co-glycolide), Ultrasound
Source:Pharmaceutical Research
Publisher:Kluwer Academic/Plenum Publ
Page Range:250-257
Date:1 January 2002
PubMed:View item in PubMed

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