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SH3GL3 associates with the Huntingtin exon 1 protein and promotes the formation of polygln-containing protein aggregates

Item Type:Article
Title:SH3GL3 associates with the Huntingtin exon 1 protein and promotes the formation of polygln-containing protein aggregates
Creators Name:Sittler, A. and Walter, S. and Wedemeyer, N. and Hasenbank, R. and Scherzinger, E. and Eickhoff, H. and Bates, G.P. and Lehrach, H. and Wanker, E.E.
Abstract:The mechanism by which aggregated polygins cause the selective neurodegeneration in Huntington's disease (HD) is unknown. Here, we show that the SH3GL3 protein, which is preferentially expressed in brain and testis, selectively interacts with the HD exon 1 protein (HDex1p) containing a glutamine repeat in the pathological range and promotes the formation of insoluble polyglutamine-containing aggregates in vivo. The C-terminal SH3 domain in SH3GL3 and the proline-rich region in HDex1p are essential for the interaction. Coimmunoprecipitations and immunofluorescence studies revealed that SH3GL3 and HDex1p colocalize in transfected COS cells. Additionally, an anti-SH3GL3 antibody was also able to coimmunoprecipitate the full-length huntingtin from an HD human brain extract. The characteristics of the interaction between SH3GL3 and huntingtin and the colocalization of the two proteins suggest that SH3GL3 could be involved in the selective neuronal cell death in HD.
Keywords:Signal Transducing Adaptor Proteins, Brain Chemistry, COS Cells, Carrier Proteins, Exons, Gene Expression, Glutamine, Huntington Disease, Nerve Tissue Proteins, Nuclear Proteins, Peptide Fragments, Precipitin Tests, Proline, Messenger RNA, Nucleic Acid Repetitive Sequences, Subcellular Fractions, Yeasts, src Homology Domains, Animals, Rabbits
Source:Molecular Cell
ISSN:1097-2765
Publisher:Cell Press (U.S.A.)
Volume:2
Number:4
Page Range:427-436
Date:October 1998
Official Publication:https://doi.org/10.1016/S1097-2765(00)80142-2
PubMed:View item in PubMed

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