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Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells

Item Type:Article
Title:Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells
Creators Name:Luo, G. and Ivics, Z. and Izsvak, Z. and Bradley, A.
Abstract:Mouse has become an increasingly important organism for modeling human diseases and for determining gene function in a mammalian context. Unfortunately, transposon-tagged mutagenesis, one of the most valuable tools for functional genomics, still is not available in this organism. On the other hand, it has long been speculated that members of the Tc1/mariner-like elements may be less dependent on host factors and, hence, can be introduced into heterologous organisms. However, this prediction has not been realized in mice. We report here the chromosomal transposition of the Sleeping Beauty (SB) element in mouse embryonic stem cells, providing evidence that it can be used as an in vivo mutagen in mice.
Keywords:Base Sequence, Cell Line, Chromosome Mapping, DNA Primers, DNA Transposable Elements, DNA-Binding Proteins, Mammalian Embryo, Mutagenesis, Stem Cells, Transposases, Animals, Mice
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:95
Number:18
Page Range:10769-10773
Date:1 September 1998
PubMed:View item in PubMed

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