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Systematic gain-of-function genetics in Drosophila

Item Type:Article
Title:Systematic gain-of-function genetics in Drosophila
Creators Name:Rorth, P. and Szabo, K. and Bailey, A. and Laverty, T. and Rehm, J. and Rubin, G.M. and Weigmann, K. and Milan, M. and Benes, V. and Ansorge, W. and Cohen, S.M.
Abstract:A modular misexpression system was used to carry out systematic gain-of-function genetic screens in Drosophila. The system is based on inducible expression of genes tagged by insertion of a P-element vector carrying a GAL4-regulated promoter oriented to transcribe flanking genomic sequences. To identify genes involved in eye and wing development, the 2300 independent lines were screened for dominant phenotypes. Among many novel genes, the screen identified known genes, including hedgehog and decapentaplegic, implicated in these processes. A genetic interaction screen for suppressors of a cell migration defect in a hypomorphic slow border cells mutant identified known genes with likely roles in tyrosine kinase signaling and control of actin cytoskeleton, among many novel genes. These studies demonstrate the ability of the modular misexpression system to identify developmentally important genes and suggest that it will be generally useful for genetic interaction screens.
Keywords:CCAAT-Enhancer-Binding Proteins, Cell Movement, DNA-Binding Proteins, Drosophila Proteins, Eye, Developmental Gene Expression Regulation, Dominant Genes, Insect Genes, Genome, Mutation, Phenotype, Saccharomyces cerevisiae Proteins, Genetic Suppression, Transcription Factors, Wing, Animals, Drosophila
Publisher:Company of Biologists
Page Range:1049-1057
Date:1 March 1998
Official Publication:http://dev.biologists.org/content/125/6/1049.abstract
PubMed:View item in PubMed

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