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Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells

Item Type:Article
Title:Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
Creators Name:Ivics, Z. and Hackett, P.B. and Plasterk, R.H. and Izsvak, Z.
Abstract:Members of the Tc1/mariner superfamily of transposons isolated from fish appear to be transpositionally inactive due to the accumulation of mutations. Molecular phylogenetic data were used to construct a synthetic transposon, Sleeping Beauty, which could be identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase binds to the inverted repeats of salmonid transposons in a substrate-specific manner, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells. Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis.
Keywords:Amino Acid Sequence, Cell Line, DNA, DNA Transposable Elements, Recombinant DNA, Enzyme Activation, Molecular Sequence Data, Recombinant Fusion Proteins, Salmonidae, Transfection, Transposases, Animals, Carps, Mice
Source:Cell
ISSN:0092-8674
Publisher:Cell Press (U.S.A.)
Volume:91
Number:4
Page Range:501-510
Date:14 November 1997
Official Publication:https://doi.org/10.1016/S0092-8674(00)80436-5
PubMed:View item in PubMed

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