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Transcriptional activation by tetracyclines in mammalian cells

Item Type:Article
Title:Transcriptional activation by tetracyclines in mammalian cells
Creators Name:Gossen, M. and Freundlieb, S. and Bender, G. and Muller, G. and Hillen, W. and Bujard, H.
Abstract:A transcriptional transactivator was developed that fuses the VP16 activation domain with a mutant Tet repressor from Escherichia coli. This transactivator requires certain tetracycline (Tc) derivatives for specific DNA binding. Thus, addition of doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold. The specificity of the Tet repressor-operator-effector interaction and the pharmacological characteristics of Tc's make this regulatory system well suited for the control of gene activities in vivo, such as in transgenic animals and possibly in gene therapy.
Keywords:Amino Acid Sequence, Doxycycline, Reporter Genes, Hela Cells, Molecular Sequence Data, Genetic Operator Regions, Recombinant Fusion Proteins, Repressor Proteins, Simplexvirus, Trans-Activators, Transcriptional Activation, Transfection, Viral Proteins
Publisher:American Association for the Advancement of Science
Page Range:1766-1769
Date:23 June 1995
Official Publication:https://doi.org/10.1126/science.7792603
PubMed:View item in PubMed

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