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Separation of origin recognition complex functions by cross-species complementation

Item Type:Article
Title:Separation of origin recognition complex functions by cross-species complementation
Creators Name:Ehrenhofer-Murray, A.E. and Gossen, M. and Pak, D.T. and Botchan, M.R. and Rine, J.
Abstract:Transcriptional silencing at the HMRa locus of Saccharomyces cerevisiae requires the function of the origin recognition complex (ORC), the replication initiator of yeast. Expression of a Drosophila melanogaster Orc2 complementary DNA in the yeast orc2-1 strain, which is defective for replication and silencing, complemented the silencing defect but not the replication defect; this result indicated that the replication and silencing functions of ORC were separable. The orc2-1 mutation mapped to the region of greatest homology between the Drosophila and yeast proteins. The silent state mediated by DmOrc2 was epigenetic; it was propagated during mitotic divisions in a relatively stable way, whereas the nonsilent state was metastable. In contrast, the silent state was erased during meiosis.
Keywords:Molecular Cloning, DNA Replication, DNA-Binding Proteins, Drosophila Proteins, Fungal Proteins, Gene Expression Regulation, Fungal Genes, Insect Genes, Genetic Complementation Test, Mutation, Origin Recognition Complex, Nucleic Acid Regulatory Sequences, Replication Origin, Repressor Proteins, Saccharomyces Cerevisiae Proteins, Temperature, Genetic Transformation, Animals, Drosophila Melanogaster
Publisher:American Association for the Advancement of Science
Page Range:1671-1674
Date:8 December 1995
Official Publication:https://doi.org/10.1126/science.270.5242.1671
PubMed:View item in PubMed

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