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High-throughput scanning of the rat genome using interspersed repetitive sequence-PCR markers

Item Type:Article
Title:High-throughput scanning of the rat genome using interspersed repetitive sequence-PCR markers
Creators Name:Gosele, C. and Hong, L. and Kreitler, T. and Rossmann, M. and Hieke, B. and Gross, U. and Kramer, M. and Himmelbauer, H. and Bihoreau, M.T. and Kwitek-Black, A.E. and Twigger, S. and Tonellato, P.J. and Jacob, H.J. and Schalkwyk, L.C. and Lindpaintner, K. and Ganten, D. and Lehrach, H. and Knoblauch, M.
Abstract:We report the establishment of a hybridization-based marker system for the rat genome based on the PCR amplification of interspersed repetitive sequences (IRS). Overall, 351 IRS markers were mapped within the rat genome. The IRS marker panel consists of 210 nonpolymorphic and 141 polymorphic markers that were screened for presence/absence polymorphism patterns in 38 different rat strains and substrains that are commonly used in biomedical research. The IRS marker panel was demonstrated to be useful for rapid genome screening in experimental rat crosses and high-throughput characterization of large-insert genomic library clones. Information on corresponding YAC clones is made available for this IRS marker set distributed over the whole rat genome. The two existing rat radiation hybrid maps were integrated by placing the IRS markers in both maps. The genetic and physical mapping data presented provide substantial information for ongoing positional cloning projects in the rat.
Keywords:Cell Line, Chromosome Mapping, Cricetinae, Genetic Markers, Genome, Inbred F344 Rats, Inbred Strains Rats, Interspersed Repetitive Sequences, Yeast Artificial Chromosomes, Animals, Rats
Source:Genomics
ISSN:0888-7543
Publisher:Academic Press (U.S.A.)
Volume:69
Number:3
Page Range:287-294
Date:1 November 2000
Official Publication:https://doi.org/10.1006/geno.2000.6352
PubMed:View item in PubMed

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