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Antigen-specific elimination of T cells induced by oligomerized hemagglutinin (HA) 306-318

Item Type:Article
Title:Antigen-specific elimination of T cells induced by oligomerized hemagglutinin (HA) 306-318
Creators Name:Falk, K. and Roetzschke, O. and Strominger, J.L.
Abstract:In a previous study we reported that oligomerized T cell epitopes 'superactivated' CD4+ T cells. These oligomers, consisting of 12-16 copies of a peptide epitope derived from the hemagglutinin protein of influenza virus (HA306-318), induced a specific T cell response in amounts as little as 5 pg/ml. We now show that the improved antigenicity of these multimerized epitopes can also be utilized to induce 'high zone tolerance'. Tolerization, similar to activation, occurred at about 3 logs lower concentration of oligomer than of peptide. HA306-318-specific T cell cultures became nonresponsive to stimulation with peptide after incubation with 0.5-5 μg/ml HA306-318 12-mer. The nonresponsiveness was accompanied by a drastic down-regulation of the TCR and by T cell elimination by apoptotic cell death. In contrast, stimulation with peptide even at 50 μg/ml led to temporary induction of anergy. Consequently, induction of tolerance with the oligomer was permanent and no recovery of the cultures was seen in recall experiments 12-14 days after high zone exposure to the 12-mer.
Keywords:Anergy, Apoptosis, Cluster, High Zone Tolerance, MHC
Source:European Journal of Immunology
Page Range:3012-3020
Date:1 October 2000
Official Publication:https://doi.org/10.1002/1521-4141(200010)30:10<3012::AID-IMMU3012>3.0.CO;2-Q
PubMed:View item in PubMed

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