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Neogenesis of cerebellar Purkinje neurons from gene-marked bone marrow cells in vivo

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Official URL:https://doi.org/10.1083/jcb.200105103
PubMed:View item in PubMed
Creators Name:Priller, J. and Persons, D.A. and Klett, F.F. and Kempermann, G. and Kreutzberg, G.W. and Dirnagl, U.
Journal Title:Journal of Cell Biology
Journal Abbreviation:J Cell Biol
Volume:155
Number:5
Page Range:733-738
Date:26 November 2001
Keywords:Bone Marrow Transplantation, Gene Transfer, Green Fluorescent Protein, Nervous System, Purkinje Cells, Animals, Mice
Abstract:The versatility of stem cells has only recently been fully recognized. There is evidence that upon adoptive bone marrow (BM) transplantation (BMT), donor-derived cells can give rise to neuronal phenotypes in the brains of recipient mice. Yet only few cells with the characteristic shape of neurons were detected 1-6 mo post-BMT using transgenic or newborn mutant mice. To evaluate the potential of BM to generate mature neurons in adult C57BL/6 mice, we transferred the enhanced green fluorescent protein (GFP) gene into BM cells using a murine stem cell virus-based retroviral vector. Stable and high level long-term GFP expression was observed in mice transplanted with the transduced BM. Engraftment of GFP-expressing cells in the brain was monitored by intravital microscopy. In a long-term follow up of 15 mo post-BMT, fully developed Purkinje neurons were found to express GFP in both cerebellar hemispheres and in all chimeric mice. GFP-positive Purkinje cells were also detected in BM chimeras from transgenic mice that ubiquitously express GFP. Based on morphologic criteria and the expression of glutamic acid decarboxylase, the newly generated Purkinje cells were functional.
ISSN:0021-9525
Publisher:Rockefeller University Press (U.S.A.)
Additional Information:Copyright (c) 2001 by The Rockefeller University Press
Item Type:Article

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