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Requirement of functional ryanodine receptor type 3 for astrocyte migration

Item Type:Article
Title:Requirement of functional ryanodine receptor type 3 for astrocyte migration
Creators Name:Matyash, M. and Matyash, V. and Nolte, C. and Sorrentino, V. and Kettenmann, H.
Abstract:Astrocyte motility plays an important role in the response of the brain to injury and during regeneration. We used two in vitro assays, a wound-healing model and a chemotaxis assay, to study mechanisms that control astrocyte motility. Ryanodine receptors (RyR), intracellular calcium-release channels, modulate intracellular Ca2+ levels, and also motility: 1) blocking RyR with antagonizing concentration of ryanodine (200 microM) strongly attenuated motility and 2) motility of astrocytes cultured from homozygous RyR type 3 knockout mice was impaired strongly compared with wild-type. In contrast, MIP-1a-induced chemotaxis was neither impaired in the presence of ryanodine nor in the cells from the knockout animals. Reverse transcription-polymerase chain reaction (RT-PCR) analysis combined with Western blotting and immunocytochemistry confirmed the expression of RyR type 3, but not type 1 or 2 in cultured and acutely isolated astrocytes. RyR in astrocytes are linked to Ca2+ signaling because the RyR agonist 4-chloro-m-cresol induced a release of Ca2+ from intracellular stores. These results indicate that astrocytes express only RyR type 3 and that this receptor is important for controlling astrocyte motility.
Keywords:Astrocytes, Biological Models, Brain, Calcium, Cell Movement, Chemotaxis, Cultured Cells, Knockout Mice, Messenger RNA, Ryanodine, Ryanodine Receptor Calcium Release Channel, Animals, Mice
Source:FASEB Journal
Publisher:Federation of American Societies for Experimental Biology
Page Range:84-86
Date:1 January 2002
PubMed:View item in PubMed

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