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Mammalian DNA methyltransferases show different subnuclear distributions

Item Type:Article
Title:Mammalian DNA methyltransferases show different subnuclear distributions
Creators Name:Margot, J.B. and Cardoso, M.C. and Leonhardt, H.
Abstract:In mammalian cells, DNA methylation patterns are precisely maintained after DNA replication with defined changes occurring during development. The major DNA methyltransferase (Dnmt1) is associated with nuclear replication sites during S-phase, which is consistent with a role in maintenance methylation. The subcellular distribution of the recently discovered de novo DNA methyltransferases, Dnmt3a and Dnmt3b, was investigated by immunofluorescence and by epitope tagging. We now show that both Dnmt3a and Dnmt3b are distributed throughout the nucleoplasm but are not associated with nuclear DNA replication sites during S-phase. These results suggest that de novo methylation by Dnmt3a and Dnmt3b occurs independently of the replication process and might involve an alternative mechanism for accessing the target DNA. The different subcellular distribution of mammalian DNA methyltransferases might thus contribute to the regulation of DNA methylation.
Keywords:DNA Methylation, DNA Methyltransferase, DNA Replication, Dnmt1, Dnmt3, PCNA, Replication Foci
Source:Journal of Cellular Biochemistry
Page Range:373-379
Date:1 January 2001
Official Publication:https://doi.org/10.1002/jcb.1236
PubMed:View item in PubMed

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