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The nuclear orphan receptor TR4 promotes proliferation of myeloid progenitor cells

Item Type:Article
Title:The nuclear orphan receptor TR4 promotes proliferation of myeloid progenitor cells
Creators Name:Koritschoner, N.P. and Madruga, J. and Knespel, S. and Blendinger, G. and Anzinger, B. and Otto, A. and Zenke, M. and Bartunek, P.
Abstract:Nuclear receptors represent key regulators in cell proliferation, differentiation, and development. Here we demonstrate that the nuclear orphan receptor TR4 is highly expressed in hematopoietic cells and tissues and have analyzed the impact of TR4 in this cell compartment. We show that TR4, when ectopically expressed in bone marrow cells via retrovirus vector, promotes proliferation of myeloid progenitor cells. Cells represent promyelocytes as judged by morphological features, expression of cell surface molecules, and specific markers like Mim-1 and CAAT/ enhancer binding protein {beta}. We also demonstrate that the growth promoting activity of TR4 is not exclusively dependent on its association with DNA, because expression of a mutated TR4 version devoid of its DNA binding domain exhibits a similar proliferative potential as wild-type TR4. In conclusion, these data position the orphan receptor TR4 as an important regulator of myeloid progenitor cell proliferation and development.
Keywords:Acetyltransferases, CCAAT-Enhancer-Binding Protein-Beta, Cell Division, DNA, Electrophoretic Mobility Shift Assay, Flow Cytometry, Fluorescence Microscopy, Gene Expression, Messenger RNA, Myeloid Progenitor Cells, Nerve Tissue Proteins, Nuclear Proteins, Polymerase Chain Reaction, Proteins, Retinoic Acid Receptors, Steroid Receptors, Thyroid Hormone Receptors, Retinoid X Receptors, Retroviridae, Sequence Deletion, Tertiary Protein Structure, Transcription Factors, Transfection, Western Blotting, Animals, Chickens, Mice
Source:Cell Growth and Differentiation
Publisher:American Association for Cancer Research
Page Range:563-572
Date:1 January 2001
Official Publication:http://cgd.aacrjournals.org/cgi/content/abstract/12/11/563
PubMed:View item in PubMed

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