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Expression of alpha1-adrenergic receptor subtypes in heart cell culture

Item Type:Article
Title:Expression of alpha1-adrenergic receptor subtypes in heart cell culture
Creators Name:Luther, H.P. and Podlowski, S. and Schulze, W. and Morwinski, R. and Buchwalow, I. and Baumann, G. and Wallukat, G.
Abstract:Three {alpha}1-AR subtypes have been cloned so far and are designated as {alpha}1a, {alpha}1b, and {alpha}1d. Organ-specific distribution pattern and subtype-specific effects are known but not fully understood. To address a cell-type specific expression pattern in the heart we investigated expression pattern of {alpha}1-AR subtypes on RNA- and protein-level in heart tissue, cultured cardiomyocytes and non-myocytes of the rat. Each {alpha}1-AR-subtype mRNA was present in neonatal and adult rat heart culture but the relative distribution pattern was significantly different. While the {alpha}1a-AR subtype is preferentially expressed in adult cardiomyocytes, the {alpha}1b-AR subtype was preferentially expressed in the non-myocyte cell fraction. The RT-PCR results were confirmed by Western-blotting ({alpha}1b) and immunocytochemical studies. Incubation with an {alpha}1-agonist (phenylephrine) for 72 h led to a significant reduction of the {alpha}1b-AR in neonatal heart cell culture on both mRNA and protein level. In contrast, incubation with an {alpha}1-antagonist (prazosin) induced a 1.6 fold upregulation of the {alpha}1a-AR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the α1-AR subtype which is specific for cell type and ontogeny of the rat heart and may be regulated by adrenergic agents.
Keywords:{Alpha}1-Adrenoceptor, Expression, Heart Cell Culture, Non-Myocytes, Receptor Subtype, Animals, Rats
Source:Molecular and Cellular Biochemistry
Page Range:69-79
Date:1 January 2001
PubMed:View item in PubMed

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