Nitric oxide synthase isoforms I, III and protein kinase-C theta in skeletal muscle fibres of normal and streptozotocin-induced diabetic rats with and without Ginkgo biloba extract treatment

Item Type:	Article
Title:	Nitric oxide synthase isoforms I, III and protein kinase-C theta in skeletal muscle fibres of normal and streptozotocin-induced diabetic rats with and without Ginkgo biloba extract treatment
Creators Name:	Punkt, K. and Zaitsev, S. and Park, J.K. and Wellner, M. and Buchwalow, I.B.
Abstract:	The expression of nitric oxide synthase (NOS) isoforms I, III and protein kinase-C{theta} (PKCθ) in rat vastus lateralis muscle was demonstrated immunohistochemically and then correlated to the physiological metabolic fibre types: SO (slow-oxidative), FOGI, FOGII (fast-oxidative glycolytic; I more glycolytic, II more oxidative), and FG (fast-glycolytic). NOS expression in muscles from different experimental groups (normal and diabetic rats, with and without Ginkgo biloba extract treatment) was assayed by Western blotting. Generally, NOS I and PKC{theta} were co-expressed in fibres with predominantly oxidative metabolism (SO, FOGII). This suggests an interplay of PKCθ and NOS I in nitric oxide production by oxidative fibres. NOS III was more highly expressed in fibres with predominantly glycolytic metabolism (FOGI, FG). A somewhat lower NOS I immunoreactivity was also found in NOS III positive fibres suggesting that NOS III and NOS I are co-expressed in these fibres. Western blotting revealed that NOS I as well as NOS III expression in the vastus lateralis muscle was down-regulated in diabetes and increased after Ginkgo biloba extract treatment. These effects may be associated with a diminished glucose uptake by myocytes of diabetic muscles and with an improved muscle function after Ginkgo biloba treatment.
Keywords:	Computer-Assisted Image Processing, Experimental Diabetes Mellitus, Fast-Twitch Muscle Fibers, Ginkgo Biloba, Immunohistochemistry, Isoenzymes, Muscle Fibers, Nitric Oxide Synthase, Nitric Oxide Synthase Type I, Nitric Oxide Synthase Type III, Oxidation-Reduction, Phytotherapy, Plant Extracts, Protein Kinase C, Slow-Twitch Muscle Fibers, Skeletal Muscle, Western Blotting, Wistar Rats, Animals, Rats
Source:	Histochemical Journal
ISSN:	0018-2214
Publisher:	Kluwer Academic Publishers
Volume:	33
Number:	4
Page Range:	213-219
Date:	1 January 2001
Official Publication:	https://doi.org/10.1023/A:1017994108174
PubMed:	View item in PubMed

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