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Astrocytes induce manganese superoxide dismutase in brain capillary endothelial cells

Item Type:Article
Title:Astrocytes induce manganese superoxide dismutase in brain capillary endothelial cells
Creators Name:Schroeter, M.L. and Mueller, S. and Lindenau, J. and Wiesner, B. and Hanisch, U.K. and Wolf, G. and Blasig, I.E.
Abstract:Astrocytes induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). O 2, generated in blood and EC, opens the BBB. Hence, high activity of superoxide dismutase (SOD) is a prerequisite for normal BBB function. Therefore, the influence of rat astrocytes on the expression of manganese (Mn)SOD in rat EC was investigated in two coculture models of the BBB, allowing either exchange of soluble factors or additionally cellular contacts. Activity, protein content and mRNA expression of endothelial MnSOD were significantly increased in both coculture models in comparison to monoulture by soluble astrocytic factors, such as cytokines. High activity of endothelial MnSOD may be considered as a further essential property of the BBB, which is induced and maintained by astrocytes.
Keywords:Astrocytes, Blood-brain Barrier, Brain capillary Endothelial Cells, Cytokines, Free Radicals, Manganese Superoxide Dismutase, Reactive Oxygen Species, Animals, Rats
Publisher:Lippincott Williams & Wilkins
Page Range:2513-2517
Date:1 January 2001
Official Publication:http://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=N&PAGE=fulltext&AN=00001756-200108080-00045&LSLINK=80&D=ovft
PubMed:View item in PubMed

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