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Distribution and transport of cholesterol in Caenorhabditis elegans

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Item Type:Article
Title:Distribution and transport of cholesterol in Caenorhabditis elegans
Creators Name:Matyash, V. and Geier, C. and Henske, A. and Mukherjee, S. and Hirsh, D. and Thiele, C. and Grant, B. and Maxfield, F.R. and Kurzchalia, T.V.
Abstract:Cholesterol transport is an essential process in all multicellular organisms. In this study we applied two recently developed approaches to investigate the distribution and molecular mechanisms of cholesterol transport in Caenorhabditis elegans. The distribution of cholesterol in living worms was studied by imaging its fluorescent analog, dehydroergosterol, which we applied to the animals by feeding. Dehydroergosterol accumulates primarily in the pharynx, nerve ring, excretory gland cell, and gut of L1-L3 larvae. Later, the bulk of dehydroergosterol accumulates in oocytes and spermatozoa. Males display exceptionally strong labeling of spermatids, which suggests a possible role for cholesterol in sperm development. In a complementary approach, we used a photoactivatable cholesterol analog to identify cholesterol-binding proteins in C. elegans. Three major and several minor proteins were found specifically cross-linked to photocholesterol after UV irradiation. The major proteins were identified as vitellogenins. rme-2 mutants, which lack the vitellogenin receptor, fail to accumulate dehydroergosterol in oocytes and embryos and instead accumulate dehydroergosterol in the body cavity along with vitellogenin. Thus, uptake of cholesterol by C. elegans oocytes occurs via an endocytotic pathway involving yolk proteins. The pathway is a likely evolutionary ancestor of mammalian cholesterol transport.
Keywords:Biological Transport, Caenorhabditis Elegans, Cholesterol, Digestive System, Egg Proteins, Polyacrylamide Gel Electrophoresis, Endocytosis, Ergosterol, Evolution, Fluorescence Microscopy, Chemical Models, Mutation, Pharynx, Polyethylene Glycols, Precipitin Tests, Cell Surface Receptors, Spermatids, Spermatocidal Agents, Spermatozoa, Sterols, Sucrose, Ultraviolet Rays, Vitellogenins, Animals
Source:Molecular Biology of the Cell
Publisher:American Society for Cell Biology
Page Range:1725-1736
Date:June 2001
Additional Information:Copyright (c) 2001 by The American Society for Cell Biology
Official Publication:http://www.molbiolcell.org/cgi/content/abstract/12/6/1725
PubMed:View item in PubMed

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