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Positron-emission tomography of vector-mediated gene expression in gene therapy for gliomas

Official URL:https://doi.org/10.1016/S0140-6736(01)05904-9
PubMed:View item in PubMed
Creators Name:Jacobs, A. and Voges, J. and Reszka, R. and Lercher, M. and Gossmann, A. and Kracht, L. and Kaestle, C. and Wagner, R. and Wienhard, K. and Heiss, W.D.
Journal Title:Lancet
Journal Abbreviation:Lancet
Volume:358
Number:9283
Page Range:727-729
Date:1 January 2001
Keywords:Antiviral Agents, Arabinofuranosyluracil, Emission-Computed Tomography, Gene Therapy, Genetic Transduction, Glioblastoma, Human Herpesvirus 1, Local Neoplasm Recurrence, Predictive Value of Tests, Thymidine Kinase, Viral Gene Expression Regulation
Abstract:In clinical gene-therapy trials for recurrent glioblastomas, transduction of the herpes simplex virus type-1 thymidine kinase (HSV-1-tk) gene with subsequent prodrug activation by ganciclovir was found to be safe, but clinical response was poor. We used positron-emission tomography (PET) with I-124-labelled 2'-fluoro-2'-deoxy-1{beta}-D-arabino-furanosyl-5-iodo-uracil ([ 124I]-FIAU) - a specific marker substrate for gene expression of HSV-1-tk - to identify the location, magnitude, and extent of vector-mediated HSV-1-tk gene expression in a phase I/II clinical trial of gene therapy for recurrent glioblastoma in five patients. The extent of HSV-1-tk gene expression seemed to predict the therapeutic response. The expression of an exogenous gene introduced by gene therapy into patients with gliomas can be monitored non-invasively by PET.
ISSN:0140-6736
Publisher:Lancet (U.K.)
Item Type:Article

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