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Patterning of muscle acetylcholine receptor gene expression in the absence of motor innervation

Official URL:https://doi.org/10.1016/S0896-6273(01)00287-2
PubMed:View item in PubMed
Creators Name:Yang, X. and Arber, S. and William, C. and Li, L. and Tanabe, Y. and Jessell, T.M. and Birchmeier, C. and Burden, S.J.
Journal Title:Neuron
Journal Abbreviation:Neuron
Volume:30
Number:2
Page Range:399-410
Date:1 January 2001
Keywords:Afferent Neurons, Agrin, Axons, Body Patterning, Cell Surface Receptors, Cholinergic Receptors, Developmental Gene Expression Regulation, Embryonic and Fetal Development, Genetic Recombination, G-Protein-Coupled Receptors, Knockout Mice, Lysophospholipid Receptors, Motor Neurons, Muscle Denervation, Neuregulins, Receptor Protein-Tyrosine Kinases, Synapses, Animals, Mice
Abstract:The patterning of skeletal muscle is thought to depend upon signals provided by motor neurons. We show that AChR gene expression and AChR clusters are concentrated in the central region of embryonic skeletal muscle in the absence of innervation. Neurally derived Agrin is dispensable for this early phase of AChR expression, but MuSK, a receptor tyrosine kinase activated by Agrin, is required to establish this AChR prepattern. The zone of AChR expression in muscle lacking motor axons is wider than normal, indicating that neural signals refine this muscle-autonomous prepattern. Neuronal Neuregulin-1, however, is not involved in this refinement process, nor indeed in synapse-specific AChR gene expression. Our results demonstrate that AChR expression is patterned in the absence of innervation, raising the possibility that similarly prepatterned muscle-derived cues restrict axon growth and initiate synapse formation.
ISSN:0896-6273
Publisher:Cell Press (U.S.A.)
Item Type:Article

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